1D SDS-PAGE analysis and capillary electrophoresis were used to validate its selective adsorption ability. A kinetic adsorption experiment was performed to investigate the speed of adsorption and the time to reach the adsorption balance.
The as-synthesized nanoparticle was characterized by X-ray diffraction, elemental analysis, thermal gravimetric analysis, infrared spectroscopy, magnetic hysteresis loop curves, scanning electronic microscopy and transmission electron microscopy. This novel magnetic adsorbent was prepared by coating a poly( N-methacryloyl- L-aspartic acid-hydroxyethyl methacrylate) shell on Fe 3O 2 nanoparticles via a coupling agent of KH-570 using the mini-emulsion polymerization method. In this study, a core–shell structured magnetic nanocomposite of Fe 3O was found to be an effective and selective adsorbent to remove highly abundant IgG from the urine of patients with proteinuria. Selective removal of IgG from the urine of patients with proteinuria using a polymer coated core–shell magnetic nanoparticle† RSC Advances ( IF 3.361), Pub Date : , DOI: 10.1039/c6ra24560a Zhifen Deng,Kai Hu,Liangliang Bi,Hang Yuan,Yanlong Chen,Shengnan Zhao,Huifang Du,Xuesheng Yuan,Yanjie Huang,Shusheng Zhang Selective removal of highly abundant proteins prior to analysis is an important issue in proteomics.
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